Reconstitution Calculator

How to Reconstitute Peptides

Reconstituting peptides is a straightforward process that requires sterile technique and proper materials. Follow this step-by-step protocol for consistent results in your research.

Materials Needed

• Lyophilized peptide vial
• Bacteriostatic water (BAC water)
• Insulin syringes (1mL / 100 unit)
• Alcohol swabs
• Clean workspace

Step-by-Step Protocol

1. Clean: Wipe the tops of both the peptide vial and BAC water vial with alcohol swabs.
2. Draw water: Using an insulin syringe, draw the desired amount of bacteriostatic water (use calculator above).
3. Add slowly: Insert the needle into the peptide vial and let the water run down the side of the glass — do NOT spray directly onto the powder.
4. Swirl gently: Roll the vial between your hands to dissolve. Never shake vigorously as this can damage the peptide chains.
5. Wait: Allow 5-10 minutes for complete dissolution. The solution should be clear.
6. Store: Refrigerate at 2-8°C. Most reconstituted peptides remain stable for 3-4 weeks.

Common Reconstitution Ratios

Peptide	Typical Vial Size	Recommended BAC Water	Concentration
BPC-157	5mg	2mL	250mcg per 0.1mL
TB-500	5mg	2mL	250mcg per 0.1mL
CJC-1295	2mg	2mL	100mcg per 0.1mL
Ipamorelin	5mg	2.5mL	200mcg per 0.1mL
Semaglutide	5mg	2mL	250mcg per 0.1mL

Storage Guidelines

Proper storage is critical for maintaining peptide potency. See our full Peptide Storage Guide for detailed protocols.

• Unreconstituted: Store lyophilized peptides at -20°C for long-term, or 2-8°C for short-term (weeks).
• Reconstituted: Always refrigerate at 2-8°C. Use within 3-4 weeks for most peptides.
• Avoid: Light exposure, repeated freeze-thaw cycles, and temperatures above 25°C.

Frequently Asked Questions

What is bacteriostatic water?

Bacteriostatic water is sterile water containing 0.9% benzyl alcohol as a preservative. It prevents bacterial growth in reconstituted peptide solutions, extending shelf life compared to sterile water.

Can I use sterile water instead?

Yes, but reconstituted peptides in sterile water must be used within 24-48 hours as there is no preservative to prevent bacterial growth. Bacteriostatic water extends this to 3-4 weeks.

How do I know the peptide is fully dissolved?

A properly reconstituted peptide solution should be completely clear with no visible particles or cloudiness. If particles remain after gentle swirling for 10 minutes, the peptide may be damaged.